GrippeCanadaV3, Main, Exploration, bibRecord, 000000

Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.

Identifieur interne : 000000 ( Main/Exploration ); suivant : 000001

Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.

Auteurs : J J Leblanc [Canada] ; M. Elsherif [Canada] ; S. Mulpuru [Canada] ; M. Warhuus [Canada] ; A. Ambrose [Canada] ; M. Andrew [Canada] ; G. Boivin [Canada] ; W. Bowie [Canada] ; A. Chit [États-Unis, Canada] ; G. Dos Santos [Belgique] ; K. Green [Canada] ; S A Halperin [Canada] ; T F Hatchette [Canada] ; B. Ibarguchi [Canada] ; J. Johnstone [Canada] ; K. Katz [Canada] ; J M Langley [Canada] ; P. Lagacé-Wiens [Canada] ; M. Loeb [Canada] ; A. Lund [Canada] ; D. Mackinnon-Cameron [Canada] ; A. Mccarthy [Canada] ; J E Mcelhaney [Canada] ; A. Mcgeer [Canada] ; A. Poirier [Canada] ; J. Powis [Canada] ; D. Richardson [Canada] ; M. Semret [Canada] ; V. Shinde [États-Unis] ; D. Smyth [Canada] ; S. Trottier [Canada] ; L. Valiquette [Canada] ; D. Webster [Canada] ; L. Ye [Canada] ; S A Mcneil [Canada]

Source :

Journal of medical microbiology [ 1473-5644 ] ; 2020.

RBID : pubmed:31264957

Descripteurs français

KwdFr :
- Adulte, Adulte d'âge moyen, Canada (épidémiologie), Femelle, Grippe humaine (), Grippe humaine (diagnostic), Grippe humaine (virologie), Grippe humaine (épidémiologie), Hospitalisation, Humains, Mâle, Réaction de polymérisation en chaine multiplex (), Sensibilité et spécificité, Techniques de diagnostic moléculaire (), Virus de la grippe A (), Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus influenza B (), Virus influenza B (génétique), Virus influenza B (isolement et purification).
MESH :
- diagnostic : Grippe humaine.
- génétique : Virus de la grippe A, Virus influenza B.
- isolement et purification : Virus de la grippe A, Virus influenza B.
- virologie : Grippe humaine.
- épidémiologie : Canada, Grippe humaine.
- Adulte, Adulte d'âge moyen, Femelle, Grippe humaine, Hospitalisation, Humains, Mâle, Réaction de polymérisation en chaine multiplex, Sensibilité et spécificité, Techniques de diagnostic moléculaire, Virus de la grippe A, Virus influenza B.
Wicri :
- geographic : Canada.

English descriptors

KwdEn :
- Adult, Canada (epidemiology), Female, Hospitalization, Humans, Influenza A virus (classification), Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza B virus (classification), Influenza B virus (genetics), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Influenza, Human (epidemiology), Influenza, Human (therapy), Influenza, Human (virology), Male, Middle Aged, Molecular Diagnostic Techniques (methods), Multiplex Polymerase Chain Reaction (methods), Sensitivity and Specificity.
MESH :
- geographic , epidemiology : Canada.
- classification : Influenza A virus, Influenza B virus.
- diagnosis : Influenza, Human.
- epidemiology : Influenza, Human.
- genetics : Influenza A virus, Influenza B virus.
- isolation & purification : Influenza A virus, Influenza B virus.
- methods : Molecular Diagnostic Techniques, Multiplex Polymerase Chain Reaction.
- therapy : Influenza, Human.
- virology : Influenza, Human.
- Adult, Female, Hospitalization, Humans, Male, Middle Aged, Sensitivity and Specificity.

Abstract

Background. The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.Methods. Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.Results. In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.Conclusions. Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.

DOI: 10.1099/jmm.0.001032
PubMed: 31264957

Affiliations:

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Le document en format XML

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<author><name sortKey="Bowie, W" sort="Bowie, W" uniqKey="Bowie W" first="W" last="Bowie">W. Bowie</name>
<affiliation wicri:level="1"><nlm:affiliation>University of British Columbia, Vancouver, BC, Canada.</nlm:affiliation>
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<author><name sortKey="Chit, A" sort="Chit, A" uniqKey="Chit A" first="A" last="Chit">A. Chit</name>
<affiliation wicri:level="2"><nlm:affiliation>Sanofi Pasteur, Swiftwater, PA, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Sanofi Pasteur, Swiftwater, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
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<affiliation wicri:level="4"><nlm:affiliation>Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
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<author><name sortKey="Dos Santos, G" sort="Dos Santos, G" uniqKey="Dos Santos G" first="G" last="Dos Santos">G. Dos Santos</name>
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<country xml:lang="fr">Belgique</country>
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<affiliation wicri:level="1"><nlm:affiliation>Present address: GSK, Wavre, Belgium.</nlm:affiliation>
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<author><name sortKey="Green, K" sort="Green, K" uniqKey="Green K" first="K" last="Green">K. Green</name>
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<country xml:lang="fr">Canada</country>
<wicri:regionArea>Mount Sinai Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
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<author><name sortKey="Halperin, S A" sort="Halperin, S A" uniqKey="Halperin S" first="S A" last="Halperin">S A Halperin</name>
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<author><name sortKey="Hatchette, T F" sort="Hatchette, T F" uniqKey="Hatchette T" first="T F" last="Hatchette">T F Hatchette</name>
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<author><name sortKey="Ibarguchi, B" sort="Ibarguchi, B" uniqKey="Ibarguchi B" first="B" last="Ibarguchi">B. Ibarguchi</name>
<affiliation wicri:level="1"><nlm:affiliation>GSK, Mississauga, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>GSK, Mississauga, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Present address: Bayer, Inc., Mississauga, Ontario, Canada.</nlm:affiliation>
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<author><name sortKey="Johnstone, J" sort="Johnstone, J" uniqKey="Johnstone J" first="J" last="Johnstone">J. Johnstone</name>
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<region type="state">Ontario</region>
</placeName>
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<author><name sortKey="Katz, K" sort="Katz, K" uniqKey="Katz K" first="K" last="Katz">K. Katz</name>
<affiliation wicri:level="1"><nlm:affiliation>North York General Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>North York General Hospital, Toronto, ON</wicri:regionArea>
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<author><name sortKey="Langley, J M" sort="Langley, J M" uniqKey="Langley J" first="J M" last="Langley">J M Langley</name>
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<country xml:lang="fr">Canada</country>
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<author><name sortKey="Lagace Wiens, P" sort="Lagace Wiens, P" uniqKey="Lagace Wiens P" first="P" last="Lagacé-Wiens">P. Lagacé-Wiens</name>
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<country xml:lang="fr">Canada</country>
<wicri:regionArea>St Boniface Hospital, Winnipeg, MB</wicri:regionArea>
<wicri:noRegion>MB</wicri:noRegion>
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<author><name sortKey="Loeb, M" sort="Loeb, M" uniqKey="Loeb M" first="M" last="Loeb">M. Loeb</name>
<affiliation wicri:level="4"><nlm:affiliation>Public Health Ontario and University of Toronto, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Public Health Ontario and University of Toronto, Toronto, ON</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
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<author><name sortKey="Lund, A" sort="Lund, A" uniqKey="Lund A" first="A" last="Lund">A. Lund</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
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<author><name sortKey="Mackinnon Cameron, D" sort="Mackinnon Cameron, D" uniqKey="Mackinnon Cameron D" first="D" last="Mackinnon-Cameron">D. Mackinnon-Cameron</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mccarthy, A" sort="Mccarthy, A" uniqKey="Mccarthy A" first="A" last="Mccarthy">A. Mccarthy</name>
<affiliation wicri:level="1"><nlm:affiliation>Ottawa Hospital General, Ottawa, Ontario, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Ottawa Hospital General, Ottawa, Ontario</wicri:regionArea>
<wicri:noRegion>Ontario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mcelhaney, J E" sort="Mcelhaney, J E" uniqKey="Mcelhaney J" first="J E" last="Mcelhaney">J E Mcelhaney</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Sciences North Research Institute, Sudbury, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Health Sciences North Research Institute, Sudbury, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mcgeer, A" sort="Mcgeer, A" uniqKey="Mcgeer A" first="A" last="Mcgeer">A. Mcgeer</name>
<affiliation wicri:level="1"><nlm:affiliation>Mount Sinai Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Mount Sinai Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Poirier, A" sort="Poirier, A" uniqKey="Poirier A" first="A" last="Poirier">A. Poirier</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre Intégré Universitaire de Santé et Services Sociaux, Quebec, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Centre Intégré Universitaire de Santé et Services Sociaux, Quebec, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Powis, J" sort="Powis, J" uniqKey="Powis J" first="J" last="Powis">J. Powis</name>
<affiliation wicri:level="1"><nlm:affiliation>Toronto East General Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Toronto East General Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Richardson, D" sort="Richardson, D" uniqKey="Richardson D" first="D" last="Richardson">D. Richardson</name>
<affiliation wicri:level="1"><nlm:affiliation>William Osler Health System, Brampton, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>William Osler Health System, Brampton, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Semret, M" sort="Semret, M" uniqKey="Semret M" first="M" last="Semret">M. Semret</name>
<affiliation wicri:level="4"><nlm:affiliation>McGill University, Montreal, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>McGill University, Montreal, QC</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shinde, V" sort="Shinde, V" uniqKey="Shinde V" first="V" last="Shinde">V. Shinde</name>
<affiliation wicri:level="2"><nlm:affiliation>GSK, King of Prussia, PA, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>GSK, King of Prussia, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
<affiliation wicri:level="2"><nlm:affiliation>Present address: Novavax Vaccines, Washington, DC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Present address: Novavax Vaccines, Washington, DC</wicri:regionArea>
<placeName><region type="state">District de Columbia</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Smyth, D" sort="Smyth, D" uniqKey="Smyth D" first="D" last="Smyth">D. Smyth</name>
<affiliation wicri:level="1"><nlm:affiliation>The Moncton Hospital, Moncton, NB, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>The Moncton Hospital, Moncton, NB</wicri:regionArea>
<wicri:noRegion>NB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Trottier, S" sort="Trottier, S" uniqKey="Trottier S" first="S" last="Trottier">S. Trottier</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre Hospitalier Universitaire de Québec, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Centre Hospitalier Universitaire de Québec, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
</affiliation>
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<author><name sortKey="Valiquette, L" sort="Valiquette, L" uniqKey="Valiquette L" first="L" last="Valiquette">L. Valiquette</name>
<affiliation wicri:level="1"><nlm:affiliation>Université de Sherbrooke, Sherbrooke, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Université de Sherbrooke, Sherbrooke, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
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<author><name sortKey="Webster, D" sort="Webster, D" uniqKey="Webster D" first="D" last="Webster">D. Webster</name>
<affiliation wicri:level="1"><nlm:affiliation>Horizon Health, Saint John, NB, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Horizon Health, Saint John, NB</wicri:regionArea>
<wicri:noRegion>NB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ye, L" sort="Ye, L" uniqKey="Ye L" first="L" last="Ye">L. Ye</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mcneil, S A" sort="Mcneil, S A" uniqKey="Mcneil S" first="S A" last="Mcneil">S A Mcneil</name>
<affiliation wicri:level="1"><nlm:affiliation>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
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</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2020">2020</date>
<idno type="RBID">pubmed:31264957</idno>
<idno type="pmid">31264957</idno>
<idno type="doi">10.1099/jmm.0.001032</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.</title>
<author><name sortKey="Leblanc, J J" sort="Leblanc, J J" uniqKey="Leblanc J" first="J J" last="Leblanc">J J Leblanc</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
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<author><name sortKey="Elsherif, M" sort="Elsherif, M" uniqKey="Elsherif M" first="M" last="Elsherif">M. Elsherif</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
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</author>
<author><name sortKey="Mulpuru, S" sort="Mulpuru, S" uniqKey="Mulpuru S" first="S" last="Mulpuru">S. Mulpuru</name>
<affiliation wicri:level="1"><nlm:affiliation>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
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</author>
<author><name sortKey="Warhuus, M" sort="Warhuus, M" uniqKey="Warhuus M" first="M" last="Warhuus">M. Warhuus</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
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</author>
<author><name sortKey="Ambrose, A" sort="Ambrose, A" uniqKey="Ambrose A" first="A" last="Ambrose">A. Ambrose</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Andrew, M" sort="Andrew, M" uniqKey="Andrew M" first="M" last="Andrew">M. Andrew</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
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</affiliation>
</author>
<author><name sortKey="Boivin, G" sort="Boivin, G" uniqKey="Boivin G" first="G" last="Boivin">G. Boivin</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre Hospitalier Universitaire de Québec, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Centre Hospitalier Universitaire de Québec, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bowie, W" sort="Bowie, W" uniqKey="Bowie W" first="W" last="Bowie">W. Bowie</name>
<affiliation wicri:level="1"><nlm:affiliation>University of British Columbia, Vancouver, BC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>University of British Columbia, Vancouver, BC</wicri:regionArea>
<wicri:noRegion>BC</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chit, A" sort="Chit, A" uniqKey="Chit A" first="A" last="Chit">A. Chit</name>
<affiliation wicri:level="2"><nlm:affiliation>Sanofi Pasteur, Swiftwater, PA, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Sanofi Pasteur, Swiftwater, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:affiliation>Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
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</affiliation>
</author>
<author><name sortKey="Dos Santos, G" sort="Dos Santos, G" uniqKey="Dos Santos G" first="G" last="Dos Santos">G. Dos Santos</name>
<affiliation wicri:level="3"><nlm:affiliation>Business & Decision Life Sciences (on behalf of GSK), Bruxelles, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Business & Decision Life Sciences (on behalf of GSK), Bruxelles</wicri:regionArea>
<placeName><settlement type="city">Bruxelles</settlement>
<region nuts="2">Région de Bruxelles-Capitale</region>
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</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Present address: GSK, Wavre, Belgium.</nlm:affiliation>
<country xml:lang="fr">Belgique</country>
<wicri:regionArea>Present address: GSK, Wavre</wicri:regionArea>
<wicri:noRegion>Wavre</wicri:noRegion>
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</author>
<author><name sortKey="Green, K" sort="Green, K" uniqKey="Green K" first="K" last="Green">K. Green</name>
<affiliation wicri:level="1"><nlm:affiliation>Mount Sinai Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Mount Sinai Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
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</author>
<author><name sortKey="Halperin, S A" sort="Halperin, S A" uniqKey="Halperin S" first="S A" last="Halperin">S A Halperin</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hatchette, T F" sort="Hatchette, T F" uniqKey="Hatchette T" first="T F" last="Hatchette">T F Hatchette</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ibarguchi, B" sort="Ibarguchi, B" uniqKey="Ibarguchi B" first="B" last="Ibarguchi">B. Ibarguchi</name>
<affiliation wicri:level="1"><nlm:affiliation>GSK, Mississauga, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>GSK, Mississauga, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Present address: Bayer, Inc., Mississauga, Ontario, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Present address: Bayer, Inc., Mississauga, Ontario</wicri:regionArea>
<wicri:noRegion>Ontario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Johnstone, J" sort="Johnstone, J" uniqKey="Johnstone J" first="J" last="Johnstone">J. Johnstone</name>
<affiliation wicri:level="4"><nlm:affiliation>Public Health Ontario and University of Toronto, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Public Health Ontario and University of Toronto, Toronto, ON</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Katz, K" sort="Katz, K" uniqKey="Katz K" first="K" last="Katz">K. Katz</name>
<affiliation wicri:level="1"><nlm:affiliation>North York General Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>North York General Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Langley, J M" sort="Langley, J M" uniqKey="Langley J" first="J M" last="Langley">J M Langley</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lagace Wiens, P" sort="Lagace Wiens, P" uniqKey="Lagace Wiens P" first="P" last="Lagacé-Wiens">P. Lagacé-Wiens</name>
<affiliation wicri:level="1"><nlm:affiliation>St Boniface Hospital, Winnipeg, MB, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>St Boniface Hospital, Winnipeg, MB</wicri:regionArea>
<wicri:noRegion>MB</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Loeb, M" sort="Loeb, M" uniqKey="Loeb M" first="M" last="Loeb">M. Loeb</name>
<affiliation wicri:level="4"><nlm:affiliation>Public Health Ontario and University of Toronto, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Public Health Ontario and University of Toronto, Toronto, ON</wicri:regionArea>
<orgName type="university">Université de Toronto</orgName>
<placeName><settlement type="city">Toronto</settlement>
<region type="state">Ontario</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Lund, A" sort="Lund, A" uniqKey="Lund A" first="A" last="Lund">A. Lund</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mackinnon Cameron, D" sort="Mackinnon Cameron, D" uniqKey="Mackinnon Cameron D" first="D" last="Mackinnon-Cameron">D. Mackinnon-Cameron</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
<wicri:noRegion>NS</wicri:noRegion>
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</author>
<author><name sortKey="Mccarthy, A" sort="Mccarthy, A" uniqKey="Mccarthy A" first="A" last="Mccarthy">A. Mccarthy</name>
<affiliation wicri:level="1"><nlm:affiliation>Ottawa Hospital General, Ottawa, Ontario, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Ottawa Hospital General, Ottawa, Ontario</wicri:regionArea>
<wicri:noRegion>Ontario</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mcelhaney, J E" sort="Mcelhaney, J E" uniqKey="Mcelhaney J" first="J E" last="Mcelhaney">J E Mcelhaney</name>
<affiliation wicri:level="1"><nlm:affiliation>Health Sciences North Research Institute, Sudbury, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Health Sciences North Research Institute, Sudbury, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Mcgeer, A" sort="Mcgeer, A" uniqKey="Mcgeer A" first="A" last="Mcgeer">A. Mcgeer</name>
<affiliation wicri:level="1"><nlm:affiliation>Mount Sinai Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Mount Sinai Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Poirier, A" sort="Poirier, A" uniqKey="Poirier A" first="A" last="Poirier">A. Poirier</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre Intégré Universitaire de Santé et Services Sociaux, Quebec, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Centre Intégré Universitaire de Santé et Services Sociaux, Quebec, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Powis, J" sort="Powis, J" uniqKey="Powis J" first="J" last="Powis">J. Powis</name>
<affiliation wicri:level="1"><nlm:affiliation>Toronto East General Hospital, Toronto, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Toronto East General Hospital, Toronto, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Richardson, D" sort="Richardson, D" uniqKey="Richardson D" first="D" last="Richardson">D. Richardson</name>
<affiliation wicri:level="1"><nlm:affiliation>William Osler Health System, Brampton, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>William Osler Health System, Brampton, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
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</author>
<author><name sortKey="Semret, M" sort="Semret, M" uniqKey="Semret M" first="M" last="Semret">M. Semret</name>
<affiliation wicri:level="4"><nlm:affiliation>McGill University, Montreal, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>McGill University, Montreal, QC</wicri:regionArea>
<orgName type="university">Université McGill</orgName>
<placeName><settlement type="city">Montréal</settlement>
<region type="state">Québec</region>
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</author>
<author><name sortKey="Shinde, V" sort="Shinde, V" uniqKey="Shinde V" first="V" last="Shinde">V. Shinde</name>
<affiliation wicri:level="2"><nlm:affiliation>GSK, King of Prussia, PA, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>GSK, King of Prussia, PA</wicri:regionArea>
<placeName><region type="state">Pennsylvanie</region>
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<affiliation wicri:level="2"><nlm:affiliation>Present address: Novavax Vaccines, Washington, DC, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Present address: Novavax Vaccines, Washington, DC</wicri:regionArea>
<placeName><region type="state">District de Columbia</region>
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</affiliation>
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<author><name sortKey="Smyth, D" sort="Smyth, D" uniqKey="Smyth D" first="D" last="Smyth">D. Smyth</name>
<affiliation wicri:level="1"><nlm:affiliation>The Moncton Hospital, Moncton, NB, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>The Moncton Hospital, Moncton, NB</wicri:regionArea>
<wicri:noRegion>NB</wicri:noRegion>
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<author><name sortKey="Trottier, S" sort="Trottier, S" uniqKey="Trottier S" first="S" last="Trottier">S. Trottier</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre Hospitalier Universitaire de Québec, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Centre Hospitalier Universitaire de Québec, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
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<author><name sortKey="Valiquette, L" sort="Valiquette, L" uniqKey="Valiquette L" first="L" last="Valiquette">L. Valiquette</name>
<affiliation wicri:level="1"><nlm:affiliation>Université de Sherbrooke, Sherbrooke, QC, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Université de Sherbrooke, Sherbrooke, QC</wicri:regionArea>
<wicri:noRegion>QC</wicri:noRegion>
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<author><name sortKey="Webster, D" sort="Webster, D" uniqKey="Webster D" first="D" last="Webster">D. Webster</name>
<affiliation wicri:level="1"><nlm:affiliation>Horizon Health, Saint John, NB, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Horizon Health, Saint John, NB</wicri:regionArea>
<wicri:noRegion>NB</wicri:noRegion>
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<author><name sortKey="Ye, L" sort="Ye, L" uniqKey="Ye L" first="L" last="Ye">L. Ye</name>
<affiliation wicri:level="1"><nlm:affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS</wicri:regionArea>
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<author><name sortKey="Mcneil, S A" sort="Mcneil, S A" uniqKey="Mcneil S" first="S A" last="Mcneil">S A Mcneil</name>
<affiliation wicri:level="1"><nlm:affiliation>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON</wicri:regionArea>
<wicri:noRegion>ON</wicri:noRegion>
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<series><title level="j">Journal of medical microbiology</title>
<idno type="eISSN">1473-5644</idno>
<imprint><date when="2020" type="published">2020</date>
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<term>Réaction de polymérisation en chaine multiplex</term>
<term>Sensibilité et spécificité</term>
<term>Techniques de diagnostic moléculaire</term>
<term>Virus de la grippe A</term>
<term>Virus influenza B</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Canada</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><b>Background</b>
. The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.<b>Methods</b>
. Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.<b>Results</b>
. In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.<b>Conclusions</b>
. Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">31264957</PMID>
<DateCompleted><Year>2020</Year>
<Month>03</Month>
<Day>13</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>03</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1473-5644</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>69</Volume>
<Issue>2</Issue>
<PubDate><Year>2020</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Journal of medical microbiology</Title>
<ISOAbbreviation>J. Med. Microbiol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.</ArticleTitle>
<Pagination><MedlinePgn>256-264</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1099/jmm.0.001032</ELocationID>
<Abstract><AbstractText><b>Background</b>
. The Serious Outcomes Surveillance Network of the Canadian Immunization Research Network (CIRN SOS) has been performing active influenza surveillance since 2009 (ClinicalTrials.gov identifier: NCT01517191). Influenza A and B viruses are identified and characterized using real-time reverse-transcriptase polymerase chain reaction (RT-PCR), and multiplex testing has been performed on a subset of patients to identify other respiratory virus aetiologies. Since both methods can identify influenza A and B, a direct comparison was performed.<b>Methods</b>
. Validated real-time RT-PCRs from the World Health Organization (WHO) to identify influenza A and B viruses, characterize influenza A viruses into the H1N1 or H3N2 subtypes and describe influenza B viruses belonging to the Yamagata or Victoria lineages. In a subset of patients, the Seeplex RV15 One-Step ACE Detection assay (RV15) kit was also used for the detection of other respiratory viruses.<b>Results</b>
. In total, 1111 nasopharyngeal swabs were tested by RV15 and real-time RT-PCRs for influenza A and B identification and characterization. For influenza A, RV15 showed 98.0 % sensitivity, 100 % specificity and 99.7 % accuracy. The performance characteristics of RV15 were similar for influenza A subtypes H1N1 and H3N2. For influenza B, RV15 had 99.2 % sensitivity, 100 % specificity and 99.8 % accuracy, with similar assay performance being shown for both the Yamagata and Victoria lineages.<b>Conclusions</b>
. Overall, the detection of circulating subtypes of influenza A and lineages of influenza B by RV15 was similar to detection by real-time RT-PCR. Multiplex testing with RV15 allows for a more comprehensive respiratory virus surveillance in hospitalized adults, without significantly compromising the reliability of influenza A or B virus detection.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>LeBlanc</LastName>
<ForeName>J J</ForeName>
<Initials>JJ</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>ElSherif</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Mulpuru</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Warhuus</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ambrose</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Andrew</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boivin</LastName>
<ForeName>G</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Centre Hospitalier Universitaire de Québec, QC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Bowie</LastName>
<ForeName>W</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>University of British Columbia, Vancouver, BC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Chit</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Sanofi Pasteur, Swiftwater, PA, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Dos Santos</LastName>
<ForeName>G</ForeName>
<Initials>G</Initials>
<AffiliationInfo><Affiliation>Business & Decision Life Sciences (on behalf of GSK), Bruxelles, Belgium.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Present address: GSK, Wavre, Belgium.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Green</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>Mount Sinai Hospital, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Halperin</LastName>
<ForeName>S A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Hatchette</LastName>
<ForeName>T F</ForeName>
<Initials>TF</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ibarguchi</LastName>
<ForeName>B</ForeName>
<Initials>B</Initials>
<AffiliationInfo><Affiliation>GSK, Mississauga, ON, Canada.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Present address: Bayer, Inc., Mississauga, Ontario, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Johnstone</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Public Health Ontario and University of Toronto, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Katz</LastName>
<ForeName>K</ForeName>
<Initials>K</Initials>
<AffiliationInfo><Affiliation>North York General Hospital, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Langley</LastName>
<ForeName>J M</ForeName>
<Initials>JM</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lagacé-Wiens</LastName>
<ForeName>P</ForeName>
<Initials>P</Initials>
<AffiliationInfo><Affiliation>St Boniface Hospital, Winnipeg, MB, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Loeb</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Public Health Ontario and University of Toronto, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Lund</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>MacKinnon-Cameron</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McCarthy</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Ottawa Hospital General, Ottawa, Ontario, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McElhaney</LastName>
<ForeName>J E</ForeName>
<Initials>JE</Initials>
<AffiliationInfo><Affiliation>Health Sciences North Research Institute, Sudbury, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McGeer</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Mount Sinai Hospital, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Poirier</LastName>
<ForeName>A</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Centre Intégré Universitaire de Santé et Services Sociaux, Quebec, QC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Powis</LastName>
<ForeName>J</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Toronto East General Hospital, Toronto, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Richardson</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>William Osler Health System, Brampton, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Semret</LastName>
<ForeName>M</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>McGill University, Montreal, QC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shinde</LastName>
<ForeName>V</ForeName>
<Initials>V</Initials>
<AffiliationInfo><Affiliation>GSK, King of Prussia, PA, USA.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Present address: Novavax Vaccines, Washington, DC, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Smyth</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>The Moncton Hospital, Moncton, NB, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Trottier</LastName>
<ForeName>S</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Centre Hospitalier Universitaire de Québec, QC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Valiquette</LastName>
<ForeName>L</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Université de Sherbrooke, Sherbrooke, QC, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Webster</LastName>
<ForeName>D</ForeName>
<Initials>D</Initials>
<AffiliationInfo><Affiliation>Horizon Health, Saint John, NB, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ye</LastName>
<ForeName>L</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Canadian Center for Vaccinology, Dalhousie University, IWK Health Centre, and Nova Scotia Health Authority, Halifax, NS, Canada.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>McNeil</LastName>
<ForeName>S A</ForeName>
<Initials>SA</Initials>
<AffiliationInfo><Affiliation>Ottawa Hospital Research Institute, University of Ottawa, Ottawa, ON, Canada.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<DataBankList CompleteYN="Y"><DataBank><DataBankName>ClinicalTrials.gov</DataBankName>
<AccessionNumberList><AccessionNumber>NCT01517191</AccessionNumber>
</AccessionNumberList>
</DataBank>
</DataBankList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D023361">Validation Study</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2019</Year>
<Month>06</Month>
<Day>28</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>J Med Microbiol</MedlineTA>
<NlmUniqueID>0224131</NlmUniqueID>
<ISSNLinking>0022-2615</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002170" MajorTopicYN="N" Type="Geographic">Canada</DescriptorName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005260" MajorTopicYN="N">Female</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006760" MajorTopicYN="N">Hospitalization</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009980" MajorTopicYN="N">Influenza A virus</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009981" MajorTopicYN="N">Influenza B virus</DescriptorName>
<QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007251" MajorTopicYN="N">Influenza, Human</DescriptorName>
<QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName>
<QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName>
<QualifierName UI="Q000628" MajorTopicYN="N">therapy</QualifierName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008875" MajorTopicYN="N">Middle Aged</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D025202" MajorTopicYN="N">Molecular Diagnostic Techniques</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D060885" MajorTopicYN="N">Multiplex Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">influenza</Keyword>
<Keyword MajorTopicYN="N">lineage</Keyword>
<Keyword MajorTopicYN="N">multiplex PCR</Keyword>
<Keyword MajorTopicYN="N">subtype</Keyword>
<Keyword MajorTopicYN="N">validation</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2019</Year>
<Month>7</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>3</Month>
<Day>14</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2019</Year>
<Month>7</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">31264957</ArticleId>
<ArticleId IdType="doi">10.1099/jmm.0.001032</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Belgique</li>
<li>Canada</li>
<li>États-Unis</li>
</country>
<region><li>District de Columbia</li>
<li>Ontario</li>
<li>Pennsylvanie</li>
<li>Québec</li>
<li>Région de Bruxelles-Capitale</li>
</region>
<settlement><li>Bruxelles</li>
<li>Montréal</li>
<li>Toronto</li>
</settlement>
<orgName><li>Université McGill</li>
<li>Université de Toronto</li>
</orgName>
</list>
<tree><country name="Canada"><noRegion><name sortKey="Leblanc, J J" sort="Leblanc, J J" uniqKey="Leblanc J" first="J J" last="Leblanc">J J Leblanc</name>
</noRegion>
<name sortKey="Ambrose, A" sort="Ambrose, A" uniqKey="Ambrose A" first="A" last="Ambrose">A. Ambrose</name>
<name sortKey="Andrew, M" sort="Andrew, M" uniqKey="Andrew M" first="M" last="Andrew">M. Andrew</name>
<name sortKey="Boivin, G" sort="Boivin, G" uniqKey="Boivin G" first="G" last="Boivin">G. Boivin</name>
<name sortKey="Bowie, W" sort="Bowie, W" uniqKey="Bowie W" first="W" last="Bowie">W. Bowie</name>
<name sortKey="Chit, A" sort="Chit, A" uniqKey="Chit A" first="A" last="Chit">A. Chit</name>
<name sortKey="Elsherif, M" sort="Elsherif, M" uniqKey="Elsherif M" first="M" last="Elsherif">M. Elsherif</name>
<name sortKey="Green, K" sort="Green, K" uniqKey="Green K" first="K" last="Green">K. Green</name>
<name sortKey="Halperin, S A" sort="Halperin, S A" uniqKey="Halperin S" first="S A" last="Halperin">S A Halperin</name>
<name sortKey="Hatchette, T F" sort="Hatchette, T F" uniqKey="Hatchette T" first="T F" last="Hatchette">T F Hatchette</name>
<name sortKey="Ibarguchi, B" sort="Ibarguchi, B" uniqKey="Ibarguchi B" first="B" last="Ibarguchi">B. Ibarguchi</name>
<name sortKey="Ibarguchi, B" sort="Ibarguchi, B" uniqKey="Ibarguchi B" first="B" last="Ibarguchi">B. Ibarguchi</name>
<name sortKey="Johnstone, J" sort="Johnstone, J" uniqKey="Johnstone J" first="J" last="Johnstone">J. Johnstone</name>
<name sortKey="Katz, K" sort="Katz, K" uniqKey="Katz K" first="K" last="Katz">K. Katz</name>
<name sortKey="Lagace Wiens, P" sort="Lagace Wiens, P" uniqKey="Lagace Wiens P" first="P" last="Lagacé-Wiens">P. Lagacé-Wiens</name>
<name sortKey="Langley, J M" sort="Langley, J M" uniqKey="Langley J" first="J M" last="Langley">J M Langley</name>
<name sortKey="Loeb, M" sort="Loeb, M" uniqKey="Loeb M" first="M" last="Loeb">M. Loeb</name>
<name sortKey="Lund, A" sort="Lund, A" uniqKey="Lund A" first="A" last="Lund">A. Lund</name>
<name sortKey="Mackinnon Cameron, D" sort="Mackinnon Cameron, D" uniqKey="Mackinnon Cameron D" first="D" last="Mackinnon-Cameron">D. Mackinnon-Cameron</name>
<name sortKey="Mccarthy, A" sort="Mccarthy, A" uniqKey="Mccarthy A" first="A" last="Mccarthy">A. Mccarthy</name>
<name sortKey="Mcelhaney, J E" sort="Mcelhaney, J E" uniqKey="Mcelhaney J" first="J E" last="Mcelhaney">J E Mcelhaney</name>
<name sortKey="Mcgeer, A" sort="Mcgeer, A" uniqKey="Mcgeer A" first="A" last="Mcgeer">A. Mcgeer</name>
<name sortKey="Mcneil, S A" sort="Mcneil, S A" uniqKey="Mcneil S" first="S A" last="Mcneil">S A Mcneil</name>
<name sortKey="Mulpuru, S" sort="Mulpuru, S" uniqKey="Mulpuru S" first="S" last="Mulpuru">S. Mulpuru</name>
<name sortKey="Poirier, A" sort="Poirier, A" uniqKey="Poirier A" first="A" last="Poirier">A. Poirier</name>
<name sortKey="Powis, J" sort="Powis, J" uniqKey="Powis J" first="J" last="Powis">J. Powis</name>
<name sortKey="Richardson, D" sort="Richardson, D" uniqKey="Richardson D" first="D" last="Richardson">D. Richardson</name>
<name sortKey="Semret, M" sort="Semret, M" uniqKey="Semret M" first="M" last="Semret">M. Semret</name>
<name sortKey="Smyth, D" sort="Smyth, D" uniqKey="Smyth D" first="D" last="Smyth">D. Smyth</name>
<name sortKey="Trottier, S" sort="Trottier, S" uniqKey="Trottier S" first="S" last="Trottier">S. Trottier</name>
<name sortKey="Valiquette, L" sort="Valiquette, L" uniqKey="Valiquette L" first="L" last="Valiquette">L. Valiquette</name>
<name sortKey="Warhuus, M" sort="Warhuus, M" uniqKey="Warhuus M" first="M" last="Warhuus">M. Warhuus</name>
<name sortKey="Webster, D" sort="Webster, D" uniqKey="Webster D" first="D" last="Webster">D. Webster</name>
<name sortKey="Ye, L" sort="Ye, L" uniqKey="Ye L" first="L" last="Ye">L. Ye</name>
</country>
<country name="États-Unis"><region name="Pennsylvanie"><name sortKey="Chit, A" sort="Chit, A" uniqKey="Chit A" first="A" last="Chit">A. Chit</name>
</region>
<name sortKey="Shinde, V" sort="Shinde, V" uniqKey="Shinde V" first="V" last="Shinde">V. Shinde</name>
<name sortKey="Shinde, V" sort="Shinde, V" uniqKey="Shinde V" first="V" last="Shinde">V. Shinde</name>
</country>
<country name="Belgique"><region name="Région de Bruxelles-Capitale"><name sortKey="Dos Santos, G" sort="Dos Santos, G" uniqKey="Dos Santos G" first="G" last="Dos Santos">G. Dos Santos</name>
</region>
<name sortKey="Dos Santos, G" sort="Dos Santos, G" uniqKey="Dos Santos G" first="G" last="Dos Santos">G. Dos Santos</name>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.
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Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.

Validation of the Seegene RV15 multiplex PCR for the detection of influenza A subtypes and influenza B lineages during national influenza surveillance in hospitalized adults.

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